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My thanks will also go to Sachinta Jayasinge and Norhaslinda for helping me in flow cytometry analysis Siti Hamimah for the western blot analysis Dr. Martin Spitaler for the microscopy examinations and histopathology group from Hammersmith campus of ICL especially Fatimah Jaafar for the interpretation of my microscopic slides and to GlaxoSmithKline staff especially Dr. Sharon Robinson and Bibi for a wonderful training in MLA testing.
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The media was removed and the cells were washed with D-PBS. One ml Trypsin-EDTA was added spread over the cells surface. Excess kratom effects on dogs TrypsinEDTA was removed prior to incubating for 1-2 minutes for detachment of the cells.
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